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Cerebellar learning properties are modulated by the CRF receptor in granular cells

Onderzoeksgroep De Zeeuw
Publicatiejaar 2018
Gepubliceerd in The Journal of neuroscience : the official journal of the Society for Neuroscience
Auteur(s) Gili Ezra-Nevo, Francesca Prestori, Francesca Locatelli, Teresa Soda, Michiel M Ten Brinke, Mareen Engel, Henk-Jan Boele, Laura Botta, Dena Leshkowitz, Assaf Ramot, Michael Tsoory, Inbal E Biton, Jan Deussing, Egidio D'Angelo, C.I. De Zeeuw, Alon Chen

Corticotropin-releasing factor (CRF) and its type 1 receptor (CRFR1) play an important role in the responses to stressful challenges. Despite the well-established expression of CRFR1 in granular cells (GrCs), its role in procedural motor performance and memory formation remains elusive. To investigate the role of CRFR1 expression in cerebellar GrCs, we used a mouse model depleted of CRFR1 in these cells. We detected changes in the cellular learning mechanisms in GrCs depleted of CRFR1 in that they showed changes in intrinsic excitability and long-term synaptic plasticity. Moreover, male mice depleted of CRFR1 specifically in GrCs showed accelerated Pavlovian associative eye-blink conditioning, but no differences in baseline motor performance, locomotion or fear and anxiety-related behaviors. Last, we analyzed cerebella transcriptome of KO and control mice and detected prominent alterations in the expression of calcium signaling pathways components. Our findings shed light on the interplay between stress-related central mechanisms and cerebellar motor conditioning, highlighting the role of the CRF system in regulating particular forms of cerebellar learning.SIGNIFICANCE STATEMENTAlthough it is known that CRFR1 is highly expressed in the cerebellum, little attention has been given to its role in cerebellar functions in the behaving animal. Moreover, most of the attention was directed to the effect of CRF on Purkinje cells at the cellular level, and to this date, almost no data exist on the role of this stress-related receptor in other cerebellar structures. Here, we explored the behavioral and cellular effect of GrCs specific ablation of CRFR1 We found a profound effect on learning, both at the cellular and behavioral levels, without affecting baseline motor skills.

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